Anti-Interleukin-1β Antibody, Alexa Fluor^™ 488 Conjugate

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IL-1beta in 4% paraformaldehyde-fixed, Triton X-100-permeabilized NIH/3T3 cells.

Research Category
Inflammation & Immunology

The unconjugated antibody (Cat. No. AB1413-I) and Alexa Fluor^™ 555 conjugate (Cat. No. AB1413-I-AF555) are also available for Immunocytochemistry, Immunoprecipitation, and Western Blotting applications.

This rabbit polyclonal Anti-Interleukin-1 Antibody, Alexa Fluor^™ 488 Conjugate, Cat. No. AB1413-I-AF488, is validated for use in Immunocytochemistry for the detection of Interleukin-1 .

The unconjugated antibody (Cat. No. AB1413-I) detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Interleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (sIL-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein.

~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to a sequence within the C-terminal half of murine and rat mature IL-1beta.

Concentration: Please refer to lot specific datasheet.

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

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