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AB15392

Anti-Zic2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Zic family member 2 (odd-paired Drosophila homolog), Zic family member 2 (odd-paired homolog, Drosophila), Zinc finger protein of the cerebellum 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology), bovine (based on 100% sequence homology), monkey (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable, western blot: suitable

NCBI accession no.

NP_033600

UniProt accession no.

Q62520

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Zic2(22772)

General description

55 kDa
Zinc-finger transcription factor (Zic2) is a conserved signaling protein with critical roles in neural development including neurulation, anteroposterior patterning, neural crest formation, cerebellar development, and ipsilateral axonal targeting across the optic chiasm. It activates the transcription of target genes by DNA binding and recruitment of RNA helicase A (RHA). Zic2 has been shown to operate as a transcriptional repressor of cellular proliferation and neurogenesis. Zic2 is expressed in pluripotent cells in the blastocyst and adult brain expression overlaps with markers of neurogenesis. The Zic2kd/kd mice show holoprosencephaly and spina bifida, which derive from malformations in the anterior and posterior ends of the neural tube, respectively. Due to its apparent role in the regulation of granule cell expansion, Zic2 expression is a useful marker for mature granule neurons in the developing cerebellum.

Immunogen

Epitope: This antibody recognizes Zic2 at the N-terminus region.
This antibody is generated from rabbits immunized with a GST-tagged recombinant protein corresponding to residues in the N-terminus of mouse Zic2.

Application

Anti-Zic2 Antibody is an antibody against Zic2 for use in WB, IH.
Immunohistochemistry Analysis: 1:200 dilution from a previous lot detected Zic2 on mouse cerebellum and hippocampus tissue.

Biochem/physiol Actions

This antibody recognizes Zic2.

Analysis Note

Evaluated by Western Blot in mouse cerebellum lysate.

Western Blot Analysis: : 0.1 µg/ml of this antibody detected Zic2 on 10 µg of mouse cerebellum lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Marije Hoogland et al.
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Regulation of prelamin A but not lamin C by miR-9, a brain-specific microRNA.
Jung, HJ; Coffinier, C; Choe, Y; Beigneux, AP; Davies, BS; Yang, SH; Barnes, RH; Hong et al.
Proceedings of the National Academy of Sciences of the USA null
Roberta De Mori et al.
Cell and tissue research, 396(2), 255-267 (2024-03-19)
Joubert syndrome (JS) is a recessively inherited congenital ataxia characterized by hypotonia, psychomotor delay, abnormal ocular movements, intellectual disability, and a peculiar cerebellar and brainstem malformation, the "molar tooth sign." Over 40 causative genes have been reported, all encoding for