Key Documents
AB15396
Anti-acetyl BMAL1 (Lys538) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
ARNT-like protein 1, brain and muscle, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Member of PAS protein 3, aryl hydrocarbon receptor nuclear translocator-like, bHLH-PAS protein JAP3, basic-helix-loop-helix-PAS orphan MOP3, mem
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
primate, opossum, mouse, rat, pig, horse
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
horse ... Arntl(100034115)
mouse ... Arntl(11865)
pig ... Arntl(100037286)
rat ... Arntl(29657)
General description
Approx. 95 kDa
Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. BMAL1 is the only component of the mammalian circadian clock whose sole deletion in a mouse model generates arrhythmicity. In addition to defects in the clock, these BMAL1 null-mice also have reproductive problems are small in stature, age quickly and have progressive arthropathy that results in having less overall locomotor activity than wild type mice. Recent phenotyping data suggests that BMAL1 and its partner Clock also play a role in regulation of glucose homeostasis and metabolism. Finally, BMAL1, NPAS2, and PER2 have been associated with seasonal affective disorder in humans. BMAL1 transcription is circadian and reciprocally regulated by NR1D1 (Rev-erb-alpha) and RORA, which establishes a second interlocking loop in the mammalian circadian clock.
Immunogen
Epitope: C-Terminus
KLH-conjugated BMAL1 acetylated at Lysine538.
Application
Anti-acetyl BMAL1 (Lys538) Antibody is an antibody against acetyl BMAL1 (Lys538) for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Circadian Rhythm & Sleep
Circadian Rhythm & Sleep
Biochem/physiol Actions
Cat. No. AB15396 recognizes acetylated lysine (Kac) within the –GG[Kac]KI LN— sequence present in BMAL1. The (Lys538) designation is based on human isoform BMAL1B (Uniprot O00327-2; also known as JAP3). The lysine numbering varies from spliced isoform to isoform, and from species to species. Note: Lys 537 mentioned in the publication by Hirayama, J. et al. (2007). Nature. 450(7172):1086-1090, in fact corresponds to Lys538 based on the Uniprot sequence O00327-2
Physical form
Antigen Affinity Purified
Antigen Affinity purified Rabbit polyclonal, supplied at 1 mg/mL, containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Transfected HEK293/myc-BMAL1/empty
vector and HEK293/myc-BMAL1/myc-CLOCK
treated with Nicotinamide.
Transfected HEK293/myc-BMAL1/empty
vector and HEK293/myc-BMAL1/myc-CLOCK
treated with Nicotinamide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Nikolai Petkau et al.
eLife, 8 (2019-07-12)
Many physiological processes exhibit circadian rhythms driven by cellular clocks composed of interlinked activating and repressing elements. To investigate temporal regulation in this molecular oscillator, we combined mouse genetic approaches and analyses of interactions of key circadian proteins with each
Shogo Sato et al.
Cell, 170(4), 664-677 (2017-08-13)
The process of aging and circadian rhythms are intimately intertwined, but how peripheral clocks involved in metabolic homeostasis contribute to aging remains unknown. Importantly, caloric restriction (CR) extends lifespan in several organisms and rewires circadian metabolism. Using young versus old
Kevin B Koronowski et al.
Cell, 177(6), 1448-1462 (2019-06-01)
Mammals rely on a network of circadian clocks to control daily systemic metabolism and physiology. The central pacemaker in the suprachiasmatic nucleus (SCN) is considered hierarchically dominant over peripheral clocks, whose degree of independence, or tissue-level autonomy, has never been
Kenichiro Kinouchi et al.
Cell reports, 25(12), 3299-3314 (2018-12-20)
The circadian clock operates as intrinsic time-keeping machinery to preserve homeostasis in response to the changing environment. While food is a known zeitgeber for clocks in peripheral tissues, it remains unclear how lack of food influences clock function. We demonstrate
Daniel C Levine et al.
Molecular cell, 78(5), 835-849 (2020-05-06)
Disrupted sleep-wake and molecular circadian rhythms are a feature of aging associated with metabolic disease and reduced levels of NAD+, yet whether changes in nucleotide metabolism control circadian behavioral and genomic rhythms remains unknown. Here, we reveal that supplementation with
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