Anti-acetyl BMAL1 (Lys538) Antibody

Approx. 95 kDa

Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. BMAL1 is the only component of the mammalian circadian clock whose sole deletion in a mouse model generates arrhythmicity. In addition to defects in the clock, these BMAL1 null-mice also have reproductive problems are small in stature, age quickly and have progressive arthropathy that results in having less overall locomotor activity than wild type mice. Recent phenotyping data suggests that BMAL1 and its partner Clock also play a role in regulation of glucose homeostasis and metabolism. Finally, BMAL1, NPAS2, and PER2 have been associated with seasonal affective disorder in humans. BMAL1 transcription is circadian and reciprocally regulated by NR1D1 (Rev-erb-alpha) and RORA, which establishes a second interlocking loop in the mammalian circadian clock.

Epitope: C-Terminus

KLH-conjugated BMAL1 acetylated at Lysine538.

Anti-acetyl BMAL1 (Lys538) Antibody is an antibody against acetyl BMAL1 (Lys538) for use in WB.

Research Category
Neuroscience

Research Sub Category
Circadian Rhythm & Sleep

Cat. No. AB15396 recognizes acetylated lysine (Kac) within the –GG[Kac]KI LN— sequence present in BMAL1. The (Lys538) designation is based on human isoform BMAL1B (Uniprot O00327-2; also known as JAP3). The lysine numbering varies from spliced isoform to isoform, and from species to species. Note: Lys 537 mentioned in the publication by Hirayama, J. et al. (2007). Nature. 450(7172):1086-1090, in fact corresponds to Lys538 based on the Uniprot sequence O00327-2

Antigen Affinity Purified

Antigen Affinity purified Rabbit polyclonal, supplied at 1 mg/mL, containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Stable for 1 year at 2-8ºC from date of receipt.

Control
Transfected HEK293/myc-BMAL1/empty
vector and HEK293/myc-BMAL1/myc-CLOCK
treated with Nicotinamide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.