AB1557
Anti-NMDAR2B Antibody, pain
serum, Chemicon®
Synonym(s):
N-methyl D-aspartate Receptor Subtype 2B, NR2B
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
26
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
suitability
not suitable for immunohistochemistry
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GRIN2B(2904)
General description
180 kDa
Immunogen
C-Terminal Fusion Protein of the NMDAR2B (Amino acids 984-1104).
Application
Anti-NMDAR2B Antibody, pain detects level of NMDAR2B & has been published & validated for use in IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blotting 1:200-1:1,000 (NMDAR2B is present in high concentrations in the hippocampus)
Immunoprecipitation using solubilized hippocampal slices. 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain lysate.
Not suggested for use in immunohistochemistry.
Note: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.
Optimal working dilutions must be determined by the end user.
Immunoprecipitation using solubilized hippocampal slices. 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain lysate.
Not suggested for use in immunohistochemistry.
Note: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes NMDAR2B. By Western blot it recognizes a 180 kDa band in rat brain membranes. Also recognizes a 70 kDa band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption of AB1557 with the C-terminal fusion protein that was used to generate the antibody.
Physical form
Rabbit antiSerum. Liquid, no preservatives.
Unpurified
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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