Key Documents
AB1722
Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2
serum, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... BCL2(596)
General description
In C. elegans, ced-3 and ced-4 were identified as genes essential for programmed cell death, while ced-9 was found to be a regulator of cell death by preventing apoptosis. The first mammalian homolog for ced-3 was described in 1988 as Bcl-2 which is involved in B-cell lymphomas (Vaux and Adams, 1988, Nature 335: 440). Bcl-2 transfected B cells were shown to be resistant towards apoptosis normally induced in B cells by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis. The bcl-2 gene codes for a 25 kDa protein. The C terminal 21 amino acids encode a stretch of hydrophobic amino acids that are important in membrane docking: Bcl-2 resides on the cytoplasmic face of the mitochondrial outer membrane, the nuclear envelop, and the endoplasmic reticulum. Deletion of the C terminus does not abrogate Bcl-2 survival function! Most Bcl-2 homologs have this hydrophobic C terminal domain, though they not necessarily are located on membranes but are cytosolic (e.g. Bax).
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Immunogen
Epitope: a.a. 68-86 of mBCL2
Peptide against mouse Bcl-2 a.a. 68-86 RTSPLRPLVATAGPALSPV
Application
Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 is an antibody against Bcl-2 for use in WB, IP, IH.
Immunoblotting: Recommended antibody dilution 1:1500-3000
Immunoprecipitation: Recommended antibody dilution 1:50
Immunohistochemistry: Recommended antibody dilution 1:600-1500
Optimal antibody dilutions must be determined by end-user.
Immunoprecipitation: Recommended antibody dilution 1:50
Immunohistochemistry: Recommended antibody dilution 1:600-1500
Optimal antibody dilutions must be determined by end-user.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Biochem/physiol Actions
AB1722 recognizes Bcl-2 alpha and beta isoforms in mouse and rat. Cross reactivity with other species is unknown
Physical form
Whole serum without preservatives
Preparation Note
Store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze thaws.
Other Notes
Replaces: 04-436
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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