Key Documents
AB1919
Anti-Integrin α4 Antibody, PhosphoSerine 988
serum, Chemicon®
Synonym(s):
CD49d, VLA-4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer988)
Gene Information
human ... ITGA4(3676)
General description
Integrin alpha4 has been implicated in the pathogenesis of diseases such as asthma, arthritis, and multiple sclerosis. Phosphorylation of the cytosolic tail of integrin alpha4 at position 988 is required for optimal alpha4beta1 integrin-mediated directed cell migration. In the absence of phosphorylation at Ser988, the cytosolic tail of integrin alpha4 binds tightly to the signaling adaptor protein, paxillin, inhibiting cell migration. Phosphorylation of alpha4 at Ser988 promotes dissociation from paxillin thereby enhancing cell migration (Han, 2003). MW is approximately 150 kDa.
Immunogen
Epitope: PhosphoSerine 988
Synthetic peptide corresponding to the cytoplasmic tail of human integrin alpha4 (a.a. 986-995) containing a phosphorylated serine at position 988. The phosphopeptide was conjugated to KLH using gluteraldehyde.
Application
ELISA: 1:10,000 (direct)
Western blot: 1:1,000 to 1:5,000. On western blots of Jurkat cell extracts the antibody recognizes a major band at 140 kDa corresponding to Integrin alpha4 as well as 3 weak bands at approximately 60 kDa that are believed to be non-specific.
Immunoprecipitation: 5 μL per 500 μg whole Jurkat cell lysate.
NOTE: the use of specific inhibitors of phosphoserine phosphatse activity such as okadaic acid or NaF is strongly recommended during the preparation of protein extracts for western blot and immunoprecipitation.
Optimal working dilutions must be determined by end user.
Western blot: 1:1,000 to 1:5,000. On western blots of Jurkat cell extracts the antibody recognizes a major band at 140 kDa corresponding to Integrin alpha4 as well as 3 weak bands at approximately 60 kDa that are believed to be non-specific.
Immunoprecipitation: 5 μL per 500 μg whole Jurkat cell lysate.
NOTE: the use of specific inhibitors of phosphoserine phosphatse activity such as okadaic acid or NaF is strongly recommended during the preparation of protein extracts for western blot and immunoprecipitation.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin α4 Antibody, PhosphoSerine 988 is validated for use in ELISA, IP, WB for the detection of Integrin α4.
Biochem/physiol Actions
Integrin alpha4 phosphorylated on serine 988. Does not react with integrin alpha4 that is not phosphorylated at serine 988 (Han, 2001).
Physical form
Liquid rabbit serum containing no preservative.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
POSITIVE CONTROL: Jurkat cells
POSITIVE CONTROL: Jurkat cells
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Oisun Jung et al.
Journal of cell science, 132(20) (2019-09-29)
When targeted by the tumor-promoting enzyme heparanase, cleaved and shed syndecan-1 (Sdc1) then couples VEGFR2 (also known as KDR) to VLA-4, activating VEGFR2 and the directed migration of myeloma cells. But how VEGFR2 activates VLA-4-mediated motility has remained unknown. We now
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