AB3000
Anti-IP3 Receptor Type II Antibody
Chemicon®, from rabbit
Synonym(s):
Inositol 1,4,5-Trisphosphate Receptor
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IF, IHC, IP, WB
Citations:
17
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, hamster, human, monkey, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITPR2(3709)
Immunogen
Synthetic peptide corresponding to the carboxy terminus of the rat Type II receptor
Application
Anti-IP3 Receptor Type II Antibody detects level of IP3 Receptor Type II & has been published & validated for use in IC, IF, IH, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Western blot: 1:50 - 1:100 (detects a 260 kDa band)
Immunofluorescence: 1:10 - 1:20
Immunoprecipitation
Immunocytochemistry: Known to give positive reactivity on AR4-2J, HL-60, COS-I, HEK-293, and RBL-2H3 cell lines.
Immunohistochemistry: Paraformaldehyde or methanol fixes are effective.
Optimal working dilutions must be determined by end user.
Immunofluorescence: 1:10 - 1:20
Immunoprecipitation
Immunocytochemistry: Known to give positive reactivity on AR4-2J, HL-60, COS-I, HEK-293, and RBL-2H3 cell lines.
Immunohistochemistry: Paraformaldehyde or methanol fixes are effective.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Rabbit anti-Type II Inositol 1,4,5-Trisphosphate Receptor (IP3R) is directed against a synthetic peptide corresponding to the carboxy terminus of the rat Type II receptor. The antibody is reactive with Type II IP3R from rat, mouse, human, hamster, and monkey.
Physical form
Affinity purified versus immobilized immunogen peptide. Liquid in PBS buffer, containing 0.05% BSA, 10% glycerol, 0.005% sodium azide. Caution: Sodium azide is hazardous. MSDS available upon request.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Mark Chalmers et al.
The Biochemical journal, 394(Pt 1), 57-66 (2005-11-09)
The size and number of IP3R (inositol 1,4,5-trisphosphate receptor) clusters located on the surface of the ER (endoplasmic reticulum) is hypothesized to regulate the propagation of Ca2+ waves in cells, but the mechanisms by which the receptors cluster are not
Robert P Brumer et al.
The Journal of physiology, 601(8), 1383-1405 (2023-03-04)
Excess consumption of carbohydrates, fat and calories leads to non-alcoholic fatty liver disease (NAFLD) and hepatic insulin resistance; these are major factors in the pathogenesis of type II diabetes. Hormones and catecholamines acting through G-protein coupled receptors (GPCRs) linked to
Shafi Kuchay et al.
Nature, 546(7659), 554-558 (2017-06-15)
In response to environmental cues that promote IP3 (inositol 1,4,5-trisphosphate) generation, IP3 receptors (IP3Rs) located on the endoplasmic reticulum allow the 'quasisynaptical' feeding of calcium to the mitochondria to promote oxidative phosphorylation. However, persistent Ca2+ release results in mitochondrial Ca2+