Anti-Aquaporin 7 Antibody

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-7 (rAQP7, 269aa) is abundantly expressed in testis. It is involved in water, glycerol and urea transport. Aquaporin-7 is also found in adipose tissue, kidney, and heart. Aquaporin-7 has been localized to in the spermatids and at maturing sperms. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

An 18 AA synthetic peptide within the N-terminal domain of rat Aquaporin-7 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.

Detect Aquaporin 7 using this Anti-Aquaporin 7 Antibody validated for use in ELISA & WB.

Note: We recommend the use of 0.5-1.0% milk in all primary/secondary antibody incubations to suppress non-specific reactivity.

Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 0.5-1.0 μg/mL when tested against 1 μg/mL of the immunogen peptide.

Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Aquaporin 7. The immunogen peptide is unique to Aquaporin-7 without significant homology to any other Aquaporins.

The peptide has 81% similarity with the mouse Aquaporin-7

Affinity Purified 1 mg/ml soln in PBS, pH 7.5 and 0.1% BSA and 0.05% sodium azide

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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