Key Documents
AB3202
Anti-LIM-3 Antibody
Chemicon®, from rabbit
Synonym(s):
Anti-HMFN1661
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
fish, frog, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ABLIM3(22885)
Immunogen
C-terminal portion of mouse LIM-3 protein.
Application
Anti-LIM-3 Antibody is an antibody against LIM-3 for use in IP, WB, IC, IH, IH(P).
Immunohistochemistry: 1:250-1:1,000 on paraffin embedded sections. 1:3,000-1:6,000 on frozen sections. 1:4,000-1:8,000 on whole mounts. Recommended fixative MEMFA.
Immunocytochemistry: 1:200-1:500
Western blot: 1:2,500-1:5,000
Immunoprecipitation: 1:200
This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:200-1:500
Western blot: 1:2,500-1:5,000
Immunoprecipitation: 1:200
This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Neuronal & Glial Markers
Developmental Neuroscience
Neuronal & Glial Markers
Biochem/physiol Actions
Recognizes LIM-3. This antibody does not appear to cross react with gsh-4 on tissue sections but may cross react in Western blot and immunoprecipitation.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mai Nakamura et al.
Cytotechnology, 68(3), 409-417 (2014-10-31)
Mouse embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the ability to differentiate in vitro into various cell lineages including neurons. The differentiation of these cells into neurons has potential applications in regenerative medicine. Previously, we reported
Clifford R Hume et al.
Gene expression patterns : GEP, 7(7), 798-807 (2007-07-03)
A cascade of transcription factors is believed to regulate the coordinate differentiation of primordial inner ear cells into the subtypes of hair cells and supporting cells. While candidate genes involved in this process have been identified, the temporal and spatial
Stephanie W Fowler et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(23), 7871-7885 (2014-06-06)
An unresolved debate in Alzheimer's disease (AD) is whether amyloid plaques are pathogenic, causing overt physical disruption of neural circuits, or protective, sequestering soluble forms of amyloid-β (Aβ) that initiate synaptic damage and cognitive decline. Few animal models of AD
Zhiyong Liu et al.
PloS one, 9(2), e89377-e89377 (2014-03-04)
Regeneration of auditory hair cells (HCs) is a promising approach to restore hearing. Recent studies have demonstrated that induced pluripotent stem cells/embryonic stem cells or supporting cells (SCs) adjacent to HCs can be converted to adopt the HC fate. However
Anne-Laure Schang et al.
Molecular endocrinology (Baltimore, Md.), 27(1), 74-91 (2012-12-06)
GATA2 transcription factor and LIM homeodomain proteins Islet1 (ISL1) and LIM homeobox 3 (LHX3) are suspected to be involved in gonadotrope cell fate and maintenance. The GnRH receptor gene (Gnrhr), crucial for gonadotrope function, is expressed in the pituitary gland
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB3202 | 04053252673559 |
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