Anti-Surfactant Protein A Antibody

Pulmonary surfactant-associated protein A1 (SP-A1, SFTPA1, PSP-A, SP-A) binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38 kDa. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Genetic variations in SP-A are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) and respiratory distress syndrome in premature infants (RDS).

~35 kDa observed

Epitope: C-type lectin domain

KLH-conjugated linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A.

Detect SP-A using this rabbit polyclonal antibody, Anti-Surfactant Protein A Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Surfactant Protein A in human lung tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Other homologies: Uniprot shows no homology to Rat.

This antibody recognizes the C-type lectin domain of Surfactant Protein A.

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human lung tissue lysate

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Surfactant Protein A in 10 µg of human lung tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB3420

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