AB5340P

Anti-Cocaine and Amphetamine Related Transcript Antibody

Name: Anti-Cocaine and Amphetamine Related Transcript Antibody
Brand: MM

Chemicon^®, from chicken

Synonym(s):

CART/CARP

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All Photos(1)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Cocaine and Amphetamine Related Transcript Antibody, Chemicon^®, from chicken

biological source

chicken

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon^®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgY

NCBI accession no.

NM_004291.2

UniProt accession no.

Q16568

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Cartpt(27220)
rat ... Cartpt(29131)

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Application

Detect Cocaine & Amphetamine Related Transcript using this Anti-Cocaine & Amphetamine Related Transcript Antibody validated for use in ELISA, WB, IH.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Reward & Addiction

Western blotting: 1-10 μg/mL using ECL.

Immunohistochemistry: It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.

ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Cocaine and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse/rat CART peptide (55-102).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chemical coding of GABA(B) receptor-immunoreactive neurones in hypothalamic regions regulating body weight.

M Bäckberg et al.

Journal of neuroendocrinology, 15(1), 1-14 (2003-01-22)

Gamma-aminobutyric acid (GABA) interacts with hypothalamic neuronal pathways regulating feeding behaviour. GABA has been reported to stimulate feeding via both ionotropic GABA(A) and metabotropic GABA(B) receptors. The functional form of the GABA(B) receptor is a heterodimer consisting of GABA(B) receptor-1

Cellular localization of GABA receptor alpha subunit immunoreactivity in the rat hypothalamus: relationship with neurones containing orexigenic or anorexigenic peptides.

M Bäckberg et al.

Journal of neuroendocrinology, 16(7), 589-604 (2004-06-25)

gamma-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in the brain, acts via two different type of GABA receptors. GABA(A) receptors are composed of five subunits that belong to eight different classes. Depending on their subunit composition, distinct pharmacological and electrophysiological

Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells.

Yu Kodani et al.

eNeuro, 9(2) (2022-04-20)

Hypothalamic melanin-concentrating hormone (MCH) neurons are important regulators of multiple physiological processes, such as sleep, feeding, and memory. Despite the increasing interest in their neuronal functions, the molecular mechanism underlying MCH neuron development remains poorly understood. We report that a

Global Trade Item Number

SKU	GTIN
AB5340P	04053252728785

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