AB5532
Anti-Nitrotyrosine Antibody
serum, Chemicon®
Synonym(s):
NO2-Tyrosine
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable
shipped in
dry ice
Gene Information
human ... NOS1(4842)
General description
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Immunogen
NO2-Tyrosine-Glutaraldehyde-BSA
Application
Anti-Nitrotyrosine Antibody is an antibody against Nitrotyrosine for use in ELISA, IH.
Immunohistochemistry: 1:1,000-1:2,500
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Biochem/physiol Actions
NO2-Tyrosine
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
NO2-Tyrosine-G-BSA 1
NO-Tyr-G-BSA 1/233
Tyr-G-BSA 1/>100,000
NO2-5HT-G-BSA 1/>100,000
NO-5HT-G-BSA 1/>100,000
NO-Cys-G-BSA 1/>100,000
NO-W-G-BSA 1/>100,000
NO-His-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
NO2-Tyrosine-G-BSA 1
NO-Tyr-G-BSA 1/233
Tyr-G-BSA 1/>100,000
NO2-5HT-G-BSA 1/>100,000
NO-5HT-G-BSA 1/>100,000
NO-Cys-G-BSA 1/>100,000
NO-W-G-BSA 1/>100,000
NO-His-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
Physical form
Rabbit antiserum. Liquid with 0.05% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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