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AB5974

Sigma-Aldrich

Anti-Dishevelled-3 Antibody

Chemicon®, from rabbit

Synonym(s):

DVL3, DSH Homolog 3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified immunoglobulin (Partially purified)

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

input

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

NCBI accession no.

NM_004423.3

UniProt accession no.

Q92997

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DVL3(1857)

General description

DVL3 is a member of multigene family that also contains DVL1 and DVL2. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL3 encodes a predicted 716 amino acid polypeptide that shows 74% nucleotide similarity with human DVL1. Similarity is particularly high in the N-terminal region and there is more divergence in the C-terminal regions.

Immunogen

Synthetic peptide corresponding to amino acids 582-596 of human and mouse disheveiled-3 protein.

Application

Anti-Dishevelled-3 Antibody detects level of Dishevelled-3 & has been published & validated for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~90 kDa. Weak reactivity is seen to dvl-1 and dvl-2 due to protein-protein interaction, and is not abolished following peptide pre-adsorption.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Disheveiled-3 protein (dvl-3). Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-3 protein.

Physical form

Format: Purified
Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots (not less than 10 mL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Analysis Note

Control
A-431 cell lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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