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Key Documents

AB9734

Sigma-Aldrich

Anti-14-3-3 ε Antibody, NT

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken, rabbit, mouse, human, sheep, rat, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_006761.3

UniProt accession no.

P62258

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... YWHAE(7531)

General description

Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s.



The 14-3-3 epsilon, C-terminal antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.

Immunogen

Synthetic peptide from the N-terminal area of sheep 14-3-3 epsilon.

Application

This Anti-14-3-3 ε Antibody, N-terminal is validated for use in WB, IH for the detection of 14-3-3 ε.
Western blot: 1:3,000 using ECL. Immunohistochemistry: 1:200-1:400 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes. ELISA Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

14-3-3 epsilon, N-terminal. By Western blot the antibody recognizes the ~33 kDa protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stéphanie Pellegrin et al.
PloS one, 7(6), e38356-e38356 (2012-06-23)
The availability of Erythropoietin (Epo) is essential for the survival of erythroid progenitors. Here we study the effects of Epo removal on primary human erythroblasts grown from peripheral blood CD34(+) cells. The erythroblasts died rapidly from apoptosis, even in the

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