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Merck

ABC130

Anti-Atg13 Antibody

serum, from rabbit

Synonym(s):

Autophagy-related protein 13

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_001136145

UniProt accession no.

O75143

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATG13(9776)
mouse ... Atg13(51897)

General description

Autophagy-related protein 13 (ATG13) is an autophagy factor necessary for autophagosome formation. ATG13 is targeted by the TOR kinase signaling pathway which regulates autophagy by controlling ATG13 phosphorylation status and regulating the ATG13-ULK1-RB1CC1 complex.
~57 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Atg13.

Application

Anti-Atg13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting: A representative lot detected Atg13 in ATG13 transfected HEK293A cell lysates (Chan, E. Y., et al. (2009). Mol Cell Biol. 29(1):157-171.).

Biochem/physiol Actions

This antibody recognizes the C-terminus of Atg13.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Non-transfected, RISC free HEK293 cell lysate and Atg13 siRNA knockdown HEK293 cell lysate.
Evaluated by Western Blotting in non-transfected, RISC free HEK293 cell lysate and Atg13 siRNA knockdown HEK293 cell lysate.

Western Blotting: A 1:1,000 dilution from a representative lot detected Atg13 in non-transfected, RISC free HEK293 cell lysate and demonstrated a loss of signal in Atg13 siRNA knockdown HEK293 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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