ABC213

Anti-phospho ULK1 Antibody (Ser777)

Name: Anti-phospho ULK1 Antibody (Ser777)
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1

Select a Size

All Photos(2)

About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

NP_033495

UniProt accession no.

O70405

shipped in

wet ice

target post-translational modification

phosphorylation (pSer777)

General description

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

~120 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ULK1 phosphorylated at Ser777.

Application

Anti-phospho ULK1 Antibody (Ser777) is a highly specific rabbit polyclonal antibody, that targets Autophagy-related protein & has been tested in western blotting & Peptide Inhibition Assay.

Evaluated by Western Blotting/Peptide Inhibition Analysis in A431 cell lysate.

Western Blotting/Peptide Inhibition Analysis: 2 µg/mL of this antibody detected phospho ULK1 (Ser777) in 10 µg of A431 cell lysate.

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho ULK1 (Ser777) in 10 µg of A431 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Porcine epidemic diarrhoea virus (PEDV) infection activates AMPK and JNK through TAK1 to induce autophagy and enhance virus replication.

Jingxiang Wang et al.

Virulence, 13(1), 1697-1712 (2022-09-29)

Autophagy plays an important role in defending against invading microbes. However, numerous viruses can subvert autophagy to benefit their replication. Porcine epidemic diarrhoea virus (PEDV) is an aetiological agent that causes severe porcine epidemic diarrhoea. How PEDV infection regulates autophagy

UBE3A deficiency-induced autophagy is associated with activation of AMPK-ULK1 and p53 pathways.

Xiaoning Hao et al.

Experimental neurology, 363, 114358-114358 (2023-02-28)

Angelman Syndrome (AS) is a neurodevelopmental disorder caused by deficiency of the maternally expressed UBE3A gene. The UBE3A proteins functions both as an E3 ligase in the ubiquitin-proteasome system (UPS), and as a transcriptional co-activator for steroid hormone receptors. Here

Hypoxia promotes noncanonical autophagy in nucleus pulposus cells independent of MTOR and HIF1A signaling.

Hyowon Choi et al.

Autophagy, 12(9), 1631-1646 (2016-06-18)

Nucleus pulposus (NP) cells reside in the avascular and hypoxic microenvironment of intervertebral discs. Importantly, many activities related to survival and function of NP cells are controlled by the HIF-family of transcription factors. We hypothesize that NP cells adapt to

Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK.

Sheng Dai et al.

Autophagy, 13(8), 1435-1451 (2017-06-15)

The drug 2-hydroxypropyl-β-cyclodextrin (HPβCD) reduces lysosomal cholesterol accumulation in Niemann-Pick disease, type C (NPC) and has been advanced to human clinical trials. However, its mechanism of action for reducing cholesterol accumulation in NPC cells is uncertain and its molecular target

Akt-mTOR hypoactivity in bipolar disorder gives rise to cognitive impairments associated with altered neuronal structure and function.

Amanda M Vanderplow et al.

Neuron, 109(9), 1479-1496 (2021-03-26)

The Akt family of kinases exerts many of its cellular effects via the activation of the mammalian target of rapamycin (mTOR) kinase through a series of intermediary proteins. Multiple lines of evidence have identified Akt-family kinases as candidate schizophrenia and

View All Related Papers

Articles

Autophagy Mitophagy in Tumor Cell Survival Death

Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.

Global Trade Item Number

SKU	GTIN
ABC213	04053252941191

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service