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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, WB
Citations:
2
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
species reactivity (predicted by homology)
human (immunogen homology)
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATG13(9776)
mouse ... Atg13(51897)
General description
Autophagy-related protein 13 (ATG13) is an autophagy factor that is involved in the preservation of cellular nutrients under starvation conditions as well as under normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. Knockdown of ATG13 inhibits autophagosome formation.
~49 kDa observed. Uniprot describes 5 isoforms ranging between ~44 kDa and ~60 kDa Uncharacterized bands may appear in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to human ATG13.
Application
Anti-ATG13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ATG13 in mouse heart tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ATG13 in mouse heart cells.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ATG13 in mouse heart cells.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat heart tissue lysate
Rat heart tissue lysate
Evaluated by Western Blotting/Peptide Inhibition Assay in rat heart tissue lysate.
Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected ATG13 in 15 µg of rat heart tissue lysate.
Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected ATG13 in 15 µg of rat heart tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABC344 | 04053252901379 |