Anti-DEK Antibody

DEK, also known as Protein DEK, and encoded by the gene DEK, is a human chromatin modification protein found in mRNA splicing-dependent complexes. DEK interacts with histones and other chromatin proteins and is a component of the B-WICH complex, a critical complex needed for proper transcription of the 5S rRNA genes and 7SL genes by RNA polymerase III which is necessary for cell growth and proliferation. DEK protein also plays an important role in cell apoptosis and high expression levels of the human DEK have been correlated with numerous human malignancies. DEK localizes to the nucleus and has been shown to be an effective biomarker in the progression of colorectal cancers.

~ 50 kDa observed. The calculated molecular weight is 43 kDa, however DEK has been shown as a ~50 kDa band in western blots (Mor-Vaknin, N., et al. (2006). Molecular and Cellular Biology. 26(24):9484-9496). Uncharacterized band(s) may appear in some lysates.

Recombinant protein corresponding to human DEK.

Anti-DEK Antibody detects level of DEK & has been published & validated for use in DEK.

Immunohistochemistry Analysis: A representative lot detected DEK in human melanoma tissue (Khodadoust, M., et al. (2009). Cancer Res. 69:6405-6413).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DEK in 10 µg of Jurkat cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.