ABD28

Anti-Rfx6 Antibody

Name: Anti-Rfx6 Antibody
Brand: MM

serum, from rabbit

Synonym(s):

DNA-binding protein RFX6, Regulatory factor X 6, Regulatory factor X domain-containing protein 1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

technique(s)

immunohistochemistry: suitable

NCBI accession no.

NP_001152861

UniProt accession no.

Q8C7R7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Rfx6(320995)

General description

102 kDa calculated. Additional isoforms at 59 and 73 kDa have also been reported.

Rfx6 (regulatory factor X 6) is a winged-helix transcription factor that is essential for development and seems to be located exclusively in the pancreatic islets. It has been associated with endoderm development, specifically, and the differentiation of islet cells. Since insulin production happens in the beta-cells of pancreatic islets, Rfx6 may be used clinically to treat diabetes. Recently, Rfx6 function has been found to change over time and gets restricted to endocrine cells at later stages. Mitchell-Riley syndrome is thought to stem from a Rfx6 mutation.

Immunogen

GST-tagged recombinant protein corresponding to mouse Rfx6.

Application

Anti-Rfx6 Antibody is a Rabbit Polyclonal Antibody for detection of Rfx6 also known as DNA-binding protein RFX6, Regulatory factor X 6 & has been validated in IHC.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Mouse embryo pancreas tissue

Evaluated by Immunohistochemistry in mouse embryo pancreas tissue.

Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Rfx6 in mouse embryo pancreas tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The dual role of RFX6 in directing β cell development and insulin production.

Jing Lu et al.

Journal of molecular endocrinology, 66(2), 129-140 (2020-12-23)

RFX6 transcription factor is believed to play a central role in directing cell development of insulin-producing pancreatic islet. RFX6 homozygous mutations cause syndromic neonatal diabetes with hypoplastic pancreas. However, RFX6 heterozygous mutations cause maturity-onset diabetes of the young (MODY) with

Transcriptional regulatory factor X6 (Rfx6) increases gastric inhibitory polypeptide (GIP) expression in enteroendocrine K-cells and is involved in GIP hypersecretion in high fat diet-induced obesity.

Suzuki, K; Harada, N; Yamane, S; Nakamura, Y; Sasaki, K; Nasteska, D; Joo, E; Shibue et al.

The Journal of Biological Chemistry null