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Merck

ABE1378

Anti-RAD18 Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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conjugate

unconjugated

antibody form

purified antibody

clone

polyclonal

purified by

using protein G

species reactivity

rabbit, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

UniProt accession no.

Q9NS91

target post-translational modification

unmodified

Quality Level

300

Analysis Note

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected RAD18 in 10 µg of U2OS cell lysate.

Application

Epitope: C-terminal region.

Biochem/physiol Actions

This polyclonal antibody detected both ubiquitinated and non-ubiquitinated human RAD18 by targeting the C-terminal region of human Rad18 (Miyase, S., et al. (2005). J. Biol. Chem. 280(1):515-524; Tateishi, S., et al. (2003). Mol. Cell. Biol. 23(2):474-481).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GST-tagged recombinant human RAD18 C-terminal fragment.
~65/75 kDa observed. Ubiquination of RAD18 can be detected (Miyase, S., et al. (2005). J. Biol. Chem. 280:515-524). Uncharacterized band(s) may appear in some lysates.

Other Notes

Human. Predicted to react with non-human primate based on 100% sequence homology.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
ABE137804055977144994

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