Anti-Thymine-DNA Glycosylase Antibody

G/T mismatch-specific thymine DNA glycosylase (EC 3.2.2.29; UniProt Q13569; also known as hTDG, Thymine-DNA glycosylase) is encoded by the TDG gene (Gene ID 6996) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. TDG is a monofunctional DNA glycosylase that excises thymine and uracil from G·T and G·U mismatched oligonucleotide substrates as well as the highly mutagenic adduct 3,N4-ethenocytosine from double-stranded DNA. TDG DNA-binding affinity is regulated by SUMOylation, which dramatically reduces its affinity toward DNA substrate and AP site. Sumoylation also potentiates the stimulatory effect of (AP)-endonuclease (APE1/HAP1) on TDG.

~55 kDa observed. 46.05 kDa calculated. 55–60 kDa unmodified and 81-86 kDa posttranslationally modified forms reported (Hardeland, U., et al. (2002). EMBO J. 21(6): 1456-1464). Uncharacterized band(s) may appear in some lysates.

Epitope: N-terminal

KLH-conjugated linear peptide corresponding to a sequence from the N-terminal region of human Thymine-DNA Glycosylase.

Anti-Thymine-DNA Glycosylase Antibody is an antibody against Thymine-DNA Glycosylase for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected Thymine-DNA Glycosylase in A431, HeLa, HUVEC, and NIH/3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Thymine-DNA Glycosylase in 10 µg of HeLa nuclear extract.

Concentration: Please refer to lot specific datasheet.

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