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Key Documents

ABE18-AF488

Sigma-Aldrich

Anti-acetyl Histone H3 (Lys9), Alexa Fluor™ 488 Conjugate Antibody

from rabbit, ALEXA FLUOR™ 488

Synonym(s):

Histone H3.1t, H3/t, H3t, H3/g, acetyl Histone H3 (Lys9)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

acetylation (Lys9)

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
17 kDa observed. Molecular weight was observed with parent ABE18.

Immunogen

Linear peptide corresponding to acetyl Histone H3 (Lys9).

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-acetyl Histone H3 (Lys9) is validated for use in Immunocytochemistry for the detection of acetyl Histone H3 (Lys9).

Physical form

Affinity purified
Purified rabbit IgG in PBS containing 15 mg/mL BSA and 0.1% Sodium Azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl Histone H3 (Lys9) in A431 cells. ABE18-AF488 is derived from its parent antibody ABE18.
Alexa Fluor™ is a registered trademark of Life Technologies.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
ABE18-AF48804053252982637

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