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Key Documents

ABE481

Sigma-Aldrich

Anti-Centrin-3 Antibody

serum, from rabbit

Synonym(s):

Centrin-3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CETN3(1070)

General description

Centrin-3 is critical to microtubule-organizing center structure and function. Centrin-3 has been linked to cell cycle, cell division and mitotic processes and is localized in the centrosome of interphase and mitotic cells.
~19 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

Recombinant protein corresponding to human Centrin-3.

Application

Detect Centrin using this rabbit polyclonal antibody, Anti-Centrin-3 Antibody validated for use in western blotting, IP, ICC & IHC.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in HeLa cells (Klein, U. R., ad Nigg, E. A. (2009). J Cell Sci. 12(Pt18):3312-321.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
RAW264.7 cell lysate
Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Centrin-3 in 10 µg of RAW264.7 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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