Anti-JMJD2D Antibody

Jumonji domain-containing protein 2D (JMJD2D) is also called Lysine-specific demethylase 4D and JmjC domain-containing histone demethylation protein 3D. JMJD2D is a histone demethylase that specifically demethylates both di- and trimethylated H3 ′Lys-9′, but not monomethylated histone H3 ′Lys-9′ residues. JMJD2D also does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′, H3 ′Lys-36′ or H4 ′Lys-20′.

~58 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

KLH-conjugated linear peptide corresponding to human JMJD2D.

Detect Jumonji domain-containing protein (JMJD) using this rabbit polyclonal antibody, Anti-JMJD2D Antibody validated for use in western blotting, IHC & IP.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected JMJD2D in human testis and liver tissues.

Immunoprecipitation Analyis: 20 µg from a representative lot detected JMJD2D in 500 µg of HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Other homologies: Bovine, Porcine, Canine, and Rhesus Macaque (93% sequence homology), Mouse (87% sequence homology), Rat (80% sequence homology).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected JMJD2D in 10 µg of HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.