Skip to Content
Merck

Key Documents

ABE603

Sigma-Aldrich

Anti-GLE1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Nucleoporin GLE1, hGLE1, GLE1-like protein

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

Q53GS7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GLE1(2733)

General description

Nucleoporin GLE1 or GLE1-like protein (GLE1L) is an mRNA export factor in both human and yeast cells and interacts with nuclear pore complex proteins NUP155 and NUPL2. GLE1 may act at a terminal step in the export of mature RNA messages to the cytoplasm. Mutations in GLE1 have been associated with lethal congenital contracture syndrome type 1 (LCCS1) and lethal arthrogryposis with anterior horn cell disease (LAAHD).
~93 kDa observed. Uniprot describes 2 isoforms at ~75 kDa and ~80 kDa Uncharacterized bands may appear in some lysate(s).

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GLE1.

Application

Detect Nucleoporin GLE1 using this rabbit polyclonal antibody, Anti-GLE1 Antibody validated for use in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected GLE1 in mouse brain tissue.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

This antibody recognizes the C-terminus of GLE1.

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate
Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected GLE1 in 15 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service