ABF1070

Anti-DRBP76/ILF3 Antibody

Name: Anti-DRBP76/ILF3 Antibody
Brand: MM

serum, from rabbit

Synonym(s):

Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, DRBP76, M-phase phosphoprotein 4, MPP4, Nuclear factor associated with dsRNA, NFAR, Nuclear factor of activated T-cells 90 kDa, NF-AT-90, Translational control protein 80, TCP

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_036350

UniProt accession no.

Q12906

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ILF3(3609)

General description

Interleukin enhancer-binding factor 3 (UniProt Q12906; also known as Double-stranded RNA-binding protein 76, DRBP76, dsRNA binding protein NFAR-2/MPP4, Interleukin enhancer binding factor 3 90kD, M-phase phosphoprotein 4, MPP4, NF-AT-90, NFAR, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, TCP80, Translational control protein 80) is encoded by the ILF3 (also known as DRBF, NF90, MPHOSPH4) gene (Gene ID 3609) in human. DRBP76 is a dsRNA binding protein (DRBP) family member capable of binding both dsRNAs and highly structured single-stranded RNA molecules through its C-terminal region. DRBP76 influences the nucleus-cytoplasm export, stability, and translation of target mRNAs via direct interaction with their 3′ UTRs. DRBP76 is also reported to play both positive and negative regulatory roles in viral replications. DRBP76 is shown to interact with the 3′-terminal stem loop (SL) structure of the single-stranded RNA genome of the dengue virus and DRBP76 knockdown decreases production of functional dengue virus in cultured cells. On the other hand, DRBP76 can interact with full-length Zaire Ebola virus (EBOV) protein VP35 in the absence of exogenous dsRNA. Viral VP35 expression redistributes DRBP76 from the nucleus to the cytoplasm in the host cells and over-expression of DRBP76 impairs the function of the EBOV transcription/replication complex.

~100 kDa A lower size band ~75 kDa may be seen in some lysates, most likely representing a smaller size variant or a non-specific band. Uncharacterized band(s) may appear in some lysates.

Immunogen

His-tagged recombinant protein corresponding to human DRBP76/ILF3.

Application

This Anti-DRBP76/ILF3 Antibody is validated for use in Western Blotting for the detection of DRBP76/ILF3.

Western Blotting Analysis: A representative lot detected DRBP76/ILF3 in the nuclear fractions prepared from the squamous cell carcinoma of the head and neck (SCCHN) cell lines PCI-15A and PCI-15B (Umemura, N., et al, (2012). Cancer Res. 72(1):45-55).
Western Blotting Analysis: A representative lot detected DRBP76/ILF3 in the nuclear fractions prepared from Wt11 (HEK293 expressing Flag-tagged wild-type TLR3) cells (Zhu, J., et al, (2010). J Immunol. 184(10):5768-5776; Sarkar, S.N., et al, (2004). Nat Struct Mol Biol. 11(11):1060-1067).

Biochem/physiol Actions

Expected to react with all 7 spliced isoforms.

Analysis Note

Evaluated by Western Blotting in MDA-MB-435 cell lysate.

Western Blotting Analysis: A 1:500 of this antibody detected DRBP76/ILF3 in 10 µg of MDA-MB-435 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ribonucleoprotein Immunoprecipitation (RIP) Analysis.

Jennifer L Martindale et al.

Bio-protocol, 10(2), e3488-e3488 (2020-01-20)

RNAs and RNA-binding proteins (RBPs) can interact dynamically in ribonucleoprotein (RNP) complexes that play important roles in controlling gene expression programs. One of the powerful ways to investigate changes in the association of RNAs with an RBP of interest is

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Post-Translational Modifications

Global Trade Item Number

SKU	GTIN
ABF1070	04055977288018

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