ABF1215-AF488
Anti-SLP76 Antibody, Alexa Fluor™ 488 Conjugate
from rabbit, ALEXA FLUOR™ 488
別名:
Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
rabbit
conjugate
ALEXA FLUOR™ 488
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
rhesus macaque (based on 100% sequence homology)
technique(s)
flow cytometry: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... LCP2(3937)
General description
60.2 kDa calculated.
Lymphocyte cytosolic protein 2 (UniProt: Q13094; also known as Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76) is encoded by the gene LCP2 (Gene ID 3937) in human. SLP-76 is a cytoplasmic adaptor phosphoprotein that contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. Its amino terminus contains an acidic region that includes a PEST domain and several tyrosine residues that are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a SH2 domain at its carboxy terminal. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind and this complex stimulates NFAT and IL-2 gene activation after TCR engagement. SLP-76 is highly expressed in spleen, thymus, and peripheral blood leukocytes and T cells. It is expressed in B cell lines at lower levels and is not detected in fibroblast and neuroblastoma cell lines. SLP-76 is reported to play a positive role in promoting T cell development and activation as well as mast cell and platelet function. Overexpression of SLP-76 is shown to enhance IL-2 transcription after TCR signaling.
Immunogen
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human SLP76.
Application
Flow Cytometry Analysis: 1:400 dilution from a representative lot detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized THP-1 human monocytic cells.
The unconjugated antibody (Cat. No. ABF1215) is also available for Western blotting application.
The unconjugated antibody (Cat. No. ABF1215) is also available for Western blotting application.
This rabbit polyclonal Anti-SLP76, Alexa Fluor™ 488 Conjugate, Cat. No. ABF1215-AF488 is validated for use in Flow Cytometry for the detection of SLP76 (lymphocyte cytosolic protein 2).
Biochem/physiol Actions
This rabbit polyclonal antibody Alexa Fluor™ 488 conjugate detected SLP76 (lymphocyte cytosolic protein 2) in Jurkat cells by flow cytometry.
Analysis Note
Evaluated by Flow Cytometry in Jurkat cells.
Flow Cytometry Analysis: A 1:400 diluution of this antibody detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized Jurkat human T lymphocytes.
Flow Cytometry Analysis: A 1:400 diluution of this antibody detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized Jurkat human T lymphocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
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ABF1215-AF488:
jan
試験成績書（COA）
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