Anti-IL-21 Antibody

Interleukin-21 (UniProt Q9HBE4; also known as IL-21, Za11) is encoded by the IL21 (also known as CVID11) gene (Gene ID 59067) in human. IL-21 is a type I four-alpha-helical bundle cytokine primarily produced by CD4+ T cells and NKT cells. The heterodimeric receptor IL-21R is dominantly expressed by B cells and shares the common cytokine receptor gamma chain (γc) with receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Studies indicate that IL-21 potently regulates both adaptive and innate immune responses and possesses widely pleiotropic functions. IL-21 has also been linked to autoimmune disorders, allergies, inflammatory diseases, as well as the pathogenesis of cancer. Human IL-21 is initially translated with an N-terminal 22 aa signal peptide sequence, which is later removed to produce the 133 aa mature IL-21.

~17 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human IL-21 sequence near the C-terminus.

Flow Cytometry Analysis: 3.0 µg of this antibody detected IL-21 in human peripheral blood mononuclear cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rabbit Polyclonal Anti-IL-21 Antibody, Cat. No. ABF184 is validated for use in Western Blotting, Flow Cytometry, for the detection of IL-21.

Expected to react with pro- and mature IL-21 of both isoform 1 and isoform 2 (IL-21iso).

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in human IL-21 transfected HEK293 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-21 in 10 µg of human IL-21 transfected HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.