Anti-SMO homolog

Smoothened homolog (UniProt: P56726; also known as SMO) is encoded by the Smo (also known as Smoh) gene (Gene ID: 319757) in mouse. SMO is a G protein-coupled receptor that associates with the patched protein (PTCH) to transduce the hedgehog′s proteins signaling. In the absence of sonic hedgehog (Shh), PTCH interacts with SMO and inhibits its activity. Shh binding to PTCH removes the inhibitory effect on SMO and allows Gli transcription to proceed. Shh signaling is required throughout embryonic development and is involved in the determination of cell fate and embryonic patterning during early vertebrate development. Disruption of Shh signaling can lead to holoprosencephaly (lack of development of forebrain in the embryo). Abnormal activation of the Shh pathway is seen in a variety of human cancers and in cancer stem cells. Loss of Smo has been linked to impaired hematopoietic stem cell renewal and diminution in the induction of chronic myelogenous leukemia (CML) by Bcr-Abl. On the other hand, constitutively active Smo is shown to cause higher proliferation of CML stem cells.

~81 kDa observed; 87.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: C-terminus

His-tagged recombinant fragment corresponding to 244 amino acids from the C-terminal half of mouse Smoothened homolog.

Detect Smoothened homolog using this rabbit polyclonal Anti-SMO homolog, Cat. No. ABS1001, validated for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Smoothened homolog in Wild Type MEFs (Courtesy of Dr. Pao-Tien Chuang from the University of California, San Francisco).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Smoothened homolog in wt MEF/ SMO KO MEF (Courtesy of Dr. Pao-Tien Chuang from the University of California, San Francisco).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Smoothened homolog in wt MEF/ SMO KO MEF (Courtesy of Dr. Pao-Tien Chuang from the University of California, San Francisco).

Immunofluorescence Analysis: A representative lot detected Smoothened homolog in Wild Type MEFS (Chen, M.H., et. al. (2009). Genes Dev. 23(16):1910-28).

Research Category
Signaling

This polyclonal antibody detects smoothened homolog in mouse embryonic fibroblasts.

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in wild type mouse embryonic fibroblasts (MEF)/SMO KO MEF.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Smoothened homolog of wild type mouse embryonic fibroblasts (MEF)/SMO KO MEF.

Concentration: Please refer to lot specific datasheet.

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