ABS1656
Anti-VPS4B (UT292)
serum, from rabbit
Synonym(s):
Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Protein SKD1, Suppressor of K(+) transport growth defect 1
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About This Item
eCl@ss:
32160702
UNSPSC Code:
12352203
NACRES:
NA.46
Product Name
Anti-VPS4B (UT292), serum, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VPS4B(9525)
Analysis Note
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected VPS4B in 10 µg of HEK293 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected VPS4B in 10 µg of HEK293 cell lysate.
Application
Detect Vacuolar protein sorting-associated protein 4B using this rabbit polyclonal Anti-VPS4B (UT292), Cat. No. ABS1656, validated for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected VPS4B immunoreactivity concentrated at the centrosome during mitosis and at the midbody during cytokinesis in 3% paraformaldehyde-fixed HeLa cells. No staining was seen in VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).
Western Blotting Analysis: A representative lot detected the VPS4B target band in lysate from untransfected, but not VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).
Western Blotting Analysis: A representative lot detected the VPS4B target band in lysate from untransfected, but not VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).
Biochem/physiol Actions
VPS4B immunostaining and target band detection by UT292 was observed only in untransfected, but not VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).
General description
Vacuolar protein sorting-associated protein 4B (EC 3.6.4.6; UniProt O75351; also known as Cell migration-inducing gene 1 protein, Protein SKD1, Suppressor of K(+) transport growth defect 1) is encoded by the VPS4B (also known as SKD1, SKD1B, VPS4-2, VPS42, MIG1) gene (Gene ID 9525) in human. SKD1 is a ubiquitously expressed protein that is involved in intracellular protein trafficking at the late endosomal protein transport step. Displays about 80% sequence homology with VPS4A. It recognizes membrane-associated ESCRT-III (endosomal sorting complex required for transport III) assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Also, redistributes the ESCRT-III components to the cytoplasm for further rounds of multi-vesicular body (MVB) sorting. In conjunction with the ESCRT machinery, it also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan. SKD1 is proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner.
Ref.: Scheuring, S et al. (2001). J. Mol. Biol. 312, 469-480.
Ref.: Scheuring, S et al. (2001). J. Mol. Biol. 312, 469-480.
~52 kDa observed. 49.30 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
His-tagged recombinant full-length human VPS4B.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
Haiyu Song et al.
Cell death and differentiation, 30(9), 2151-2166 (2023-08-19)
The centrosome assembles a bipolar spindle for faithful chromosome segregation during mitosis. To prevent the inheritance of DNA damage, the DNA damage response (DDR) triggers programmed spindle multipolarity and concomitant death in mitosis through a poorly understood mechanism. We identified
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABS1656 | 04054839135293 |
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