AC113
Anti-Neomycin Phosphotransferase II Antibody | AC113
from rabbit, purified by affinity chromatography
Synonym(s):
Aminoglycoside 3′-phosphotransferase, APH(3′)-II, APH(3′)II, Kanamycin kinase, type II, Neomycin-kanamycin phosphotransferase type II
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB
Citations:
5
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
~29 kDa observed.
Immunogen
Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.
Application
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
Biochem/physiol Actions
This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).
Physical form
Affinity purified
Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).
Legal Information
Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kumi Harada et al.
International journal of molecular sciences, 17(11) (2016-11-10)
In the endoplasmic reticulum (ER), misfolded and unfolded proteins are eliminated by a process called ER-associated protein degradation (ERAD) in order to maintain cell homeostasis. In the ERAD pathway, several ER-localized E3 ubiquitin ligases target ERAD substrate proteins for ubiquitination
Saleh Bhar et al.
Human mutation, 41(11), 1918-1930 (2020-08-14)
Diamond-Blackfan anemia (DBA) is a ribosomopathy of variable expressivity and penetrance characterized by red cell aplasia, congenital anomalies, and predisposition to certain cancers, including early-onset colorectal cancer (CRC). DBA is primarily caused by a dominant mutation of a ribosomal protein
Novel proteasome assembly chaperone mutations in PSMG2/PAC2 cause the autoinflammatory interferonopathy CANDLE/PRAAS4.
Adriana A de Jesus et al.
The Journal of allergy and clinical immunology, 143(5), 1939-1943 (2019-01-22)