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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
3B8, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3B8, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TNS4(84951)
General description
Purified mouse monoclonal antibody. Recognizes the ~52 kDa TNS4 protein.
Recognizes the ~52 kDa TNS4 protein in HeLa cells.
This Anti-TNS4 Mouse mAb (3B8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of TNS4.
Immunogen
Full-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein
Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
HeLa cells
HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Sakashita, K., et al. 2008. Ann Surg Oncol.15, 2606.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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