Key Documents
AP192SA6
Donkey Anti-Mouse IgG, Alexa Fluor™ 647 Conjugate, Species Adsorbed: H, R, B, Eq, Gt, Sh, Rb, Ch, Gp Antibody
Chemicon®
Sign Into View Organizational & Contract Pricing
Select a Size
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
donkey
Quality Level
conjugate
ALEXA FLUOR™ 647
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
packaging
pkg of 0.5 mg
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
shipped in
wet ice
General description
Alexa Fluor™ 647-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (minimal cross-reaction to Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins).
Immunogen
Fluorophore Information
Alexa Fluor™ 647 carboxylic acid
Amax=651; Emax=667
Fluorophore/Protein Absorbance Ratio: A651/A280=4.5
Alexa Fluor™ 647 carboxylic acid
Amax=651; Emax=667
Fluorophore/Protein Absorbance Ratio: A651/A280=4.5
Application
Donkey anti-Mouse IgG, Alexa Fluor 647 Conjugate, Species Adsorbed: H, R, B, Eq, Gt, Sh, Rb, Ch, Gp Antibody is an antibody against Mouse IgG for use in Immunofluorescence.
Reconstitute the product to 1.4 mg/mL with dH2O.
Suggested dilution range for most applications: 1:100 – 1:800.
Optimal working dilutions must be determined by the end user.
Suggested dilution range for most applications: 1:100 – 1:800.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
Biochem/physiol Actions
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species.
Physical form
Lyophilized in buffer containing 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA and 0.05% sodium azide.
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Preparation Note
Stable for 1 year at 2-8ºC lyophilized from date of receipt.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Yongliang Wang et al.
Journal of biophotonics, 12(5), e201800351-e201800351 (2018-11-30)
Nucleases are important enzymes that cleave nucleic acids and play critical roles in DNA repair, immune defense and potentially in cancer invasion. However, their spatial dynamics at subcellular level is much less studied. Here, we developed a surface-tethered nuclease sensor
Chencheng Feng et al.
International journal of molecular medicine, 41(6), 3316-3326 (2018-03-08)
Intervertebral disc (IVD) degeneration (IDD) is a widely recognized contributor to low back pain. Mechanical stress is a crucial etiological factor of IDD. During the process of IDD, a vicious circle is formed between abnormal mechanical stress and the damage
Chencheng Feng et al.
Oxidative medicine and cellular longevity, 2017, 7426458-7426458 (2017-11-18)
Senescence is a crucial driver of intervertebral disc degeneration (IDD). Disc cells are exposed to high oxygen tension due to neovascularization in degenerative discs. However, the effect of oxygen tension on disc cell senescence was unknown. Herein, rat nucleus pulposus
Yongliang Wang et al.
Biosensors & bioelectronics, 100, 192-200 (2017-09-16)
Integrin-transmitted cellular forces are critical for platelet adhesion, activation, aggregation and contraction during hemostasis and thrombosis. Measuring and mapping single platelet forces are desired in both research and clinical applications. Conventional force-to-strain based cell traction force microscopies have low resolution
Anwesha Sarkar et al.
Physical biology, 15(6), 065002-065002 (2018-05-23)
Integrin-transmitted cellular forces are crucial mechanical signals regulating a vast range of cell functions. Although various methods have been developed to visualize and quantify cellular forces at the cell-matrix interface, a method with high performance and low technical barrier is
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AP192SA6 | 04053252697517 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service