Donkey Anti-Mouse IgG, Alexa Fluor^™ 647 Conjugate, Species Adsorbed: H, R, B, Eq, Gt, Sh, Rb, Ch, Gp Antibody

Alexa Fluor^™ 647-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (minimal cross-reaction to Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins).

Fluorophore Information
Alexa Fluor^™ 647 carboxylic acid
Amax=651; Emax=667
Fluorophore/Protein Absorbance Ratio: A651/A280=4.5

Donkey anti-Mouse IgG, Alexa Fluor 647 Conjugate, Species Adsorbed: H, R, B, Eq, Gt, Sh, Rb, Ch, Gp Antibody is an antibody against Mouse IgG for use in Immunofluorescence.

Reconstitute the product to 1.4 mg/mL with dH2O.
Suggested dilution range for most applications: 1:100 – 1:800.
Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species.

Lyophilized in buffer containing 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA and 0.05% sodium azide.

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Stable for 1 year at 2-8ºC lyophilized from date of receipt.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

ALEXA FLUOR is a trademark of Life Technologies

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.