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Key Documents

CB1039

Sigma-Aldrich

Anti-S100A4 Mouse mAb (1F12-1G7)

liquid, clone 1F12-1G7, Calbiochem®

Synonym(s):

Anti-S100 Calcium Binding Protein A4

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1F12-1G7, monoclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... S100A4(6275)
mouse ... S100A4(20198)

General description

Purified mouae monoclonal antibody. Recognizes the ~10 kDa S100A4 protein.
Recognizes the ~10 kDa S100A4 protein in HeLa and NIH3T3 cells.
This Anti-S100A4 Mouse mAb (1F12-1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of S100A4.

Immunogen

Full-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
HeLa cells, NIH3T3 cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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