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About This Item
UNSPSC Code:
12352203
Clone:
1F12-1G7, monoclonal
Species reactivity:
mouse, human
Application:
—
Citations:
-
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
1F12-1G7, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... S100A4(6275)
mouse ... S100A4(20198)
General description
Purified mouae monoclonal antibody. Recognizes the ~10 kDa S100A4 protein.
Recognizes the ~10 kDa S100A4 protein in HeLa and NIH3T3 cells.
This Anti-S100A4 Mouse mAb (1F12-1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of S100A4.
Immunogen
Full-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
HeLa cells, NIH3T3 cells
HeLa cells, NIH3T3 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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