CBL251

Anti-Epithelial Specific Antigen Antibody, clone VU-1D9

Name: Anti-Epithelial Specific Antigen Antibody, clone VU-1D9
Brand: MM

clone VU-1D9, from mouse

Synonym(s):

Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastro

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All Photos(3)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Epithelial Specific Antigen Antibody, clone VU-1D9, clone VU-1D9, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

VU-1D9, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_002345

UniProt accession no.

P16422

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Control
MCF7 cell lyasate

Evaluated by Western Blotting in MCF7 cell lyate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.

Application

Anti-Epithelial Specific Antigen Antibody, clone VU-1D9 is a highly specific mouse monoclonal antibody, that targets Epithelial Cell Marker & has been tested in western blotting, ICC & IHC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Epithelial Specific Antigen in A431 cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epithelial specific antigen, also known as epithelial cell adhesion molecule (Ep-CAM) and epithelial glycoprotein (EGP), may act as an interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Epithelial specific antigen plays a role in embryonic stem cells proliferation and differentiation, and up-regulates the expression of FABP5, MYC, and cyclins A and E. Epithelial specific antigen is highly and selectively expressed by undifferentiated embryonic stem cells (ESCs). Protein levels rapidly diminish as soon as ESCs differentiate. Also expressed in most epithelial cell membranes and on the surface of adenocarcinoma.

~40 kDa observed. The calculated molecular weight of this protein is 35 kDa; however, this protein will run higher due to glycosylation.

Immunogen

NCI-H69 whole cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nuclear EpICD expression and its role in hepatocellular carcinoma.

Shin Young Park et al.

Oncology reports, 36(1), 197-204 (2016-05-14)

Regulated intramembrane proteolysis of epithelial cell adhesion molecule (EpCAM) results in shedding of the extracellular domain (EpEX) and release of the intra-cellular domain (EpICD) into the cytoplasm. Released EpICD associates with FHL2, β-catenin and Lef-1 to form a nuclear complex

Canonical and noncanonical regulatory roles for JAK2 in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and idiopathic pulmonary fibrosis.

Shaohua Wang et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(6), e22336-e22336 (2022-05-07)

Idiopathic pulmonary fibrosis (IPF) and rheumatoid arthritis-associated interstitial lung disease (RA-ILD) are two fibrotic interstitial lung diseases that share the usual interstitial pneumonia (UIP) injury pattern. Here, we report that RNA sequencing of lung biopsies from patients with RA-ILD and

Global Trade Item Number

SKU	GTIN
CBL251	04053252280955

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