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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BRIC 216, monoclonal
Species reactivity:
human
Application:
FACS, IHC (p), IP, WB
Citations:
5
Product Name
Anti-CD55 Antibody, clone BRIC 216, clone BRIC 216, Chemicon®, from mouse
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
BRIC 216, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... CD55(1604)
Immunogen
Human fibroblast cell line
Application
Anti-CD55 Antibody, clone BRIC 216 is an antibody against CD55 for use in FC, IP, WB, IH, IH(P).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Western blot: Non-reduced erythrocyte membrane prep; antigen is sialidase and trypsin resistant, pronase, chymotrypsin and AET sensitive
Flow Cytometry
Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded sections. Protease antigen retreival is recommended.
Immunoprecipitation
Inhibition studies
Optimal working dilutions must be determined by the end user.
Flow Cytometry
Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded sections. Protease antigen retreival is recommended.
Immunoprecipitation
Inhibition studies
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
This antibody recognizes the CD55 antigen a 70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). The antigen is present on erthrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. It is found specifically at the placental fetal-maternal interface.
Physical form
Format: Purified
Protein G Purified
The immunoglobulin was purified from cell culture supernatant by affinity chromatography using protein G and is presented in 1 M Tris-buffered saline, pH 7.5, containing no preservatives. The antibody has been sterile-filtered.
Preparation Note
Aliquot antibody into small volumes and store at -20°C for up to 12 months from the date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor.
Coyne, K E, et al.
Journal of immunology (Baltimore, Md. : 1950), 149, 2906-2913 (1992)
Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.
Holmes, C H, et al.
Journal of immunology (Baltimore, Md. : 1950), 144, 3099-3105 (1990)
Kyle A Carver et al.
Investigative ophthalmology & visual science, 57(2), 360-371 (2016-02-05)
Oxidative stress is a major factor involved in retinal pigment epithelium (RPE) apoptosis that underlies AMD. Drusen, extracellular lipid- and protein-containing deposits, are strongly associated with the development of AMD. Cell-derived microparticles (MPs) are small membrane-bound vesicles shed from cells.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CBL511 | 04053252738050 |