CP06
Anti-α-Tubulin Mouse mAb (DM1A)
liquid, clone DM1A, Calbiochem®
Synonym(s):
Mouse Anti-α-Tubulin, α-Tubulin Detection Antibody, Anti-Tubulin DM1A
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
DM1A, monoclonal
Species reactivity:
human, mouse, gerbil, chicken, rat
Application:
—
Citations:
658
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DM1A, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, gerbil, chicken, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (20-100 ng/mL, colorimetric or chemiluminescence;
Immunofluorescence (5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TUBA1A(7846)
General description
Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.
Immunogen
Chicken
native chick brain microtubules
Application
Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)
Immunofluorescence (5 µg/ml)
Immunofluorescence (5 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
trp E (Ab-1)
trp E (Ab-1)
Positive Control
Any cell line
Any cell line
Other Notes
Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Paulina Podkalicka et al.
Biomolecules, 10(1) (2020-01-23)
Elevated expression of heme oxygenase-1 (HO-1, encoded by HMOX1) is observed in various types of tumors. Hence, it is suggested that HO-1 may serve as a potential target in anticancer therapies. A novel approach to inhibit HO-1 is related to
Marco Kremp et al.
Frontiers in cell and developmental biology, 8, 251-251 (2020-05-21)
Proteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report
L Francisco Lorenzo-Martín et al.
Oncogene, 38(2), 209-227 (2018-08-09)
The bidirectional regulation of epithelial-mesenchymal transitions (EMT) is key in tumorigenesis. Rho GTPases regulate this process via canonical pathways that impinge on the stability of cell-to-cell contacts, cytoskeletal dynamics, and cell invasiveness. Here, we report that the Rho GTPase activators