DR1061

Anti-ENO1 Mouse mAb (8G8)

Name: Anti-ENO1 Mouse mAb (8G8)
Brand: MM

liquid, clone 8G8, Calbiochem^®

Synonym(s):

Anti-α Enolase 1

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Anti-ENO1 Mouse mAb (8G8), liquid, clone 8G8, Calbiochem^®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

8G8, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (1 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ENO1(2023)

Analysis Note

Positive Control
MCF-7 cells, Human lymphoma tissue, HeLa cells

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (1 g/ml)

Disclaimer

Toxicity: Regulatory Review (Z)

General description

Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).

Purified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.

Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.

Immunogen

Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Ho, J.A., et al. 2010. Anal. Chem.82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.

Physical form

In PBS, pH 7.30.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c