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Merck

DR1073

Anti-HMGA2 Mouse mAb (2D10)

liquid, clone 2D10, Calbiochem®

Synonym(s):

Anti-High Mobility Group AT-HOOK 2

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About This Item

UNSPSC Code:
12352203

Product Name

Anti-HMGA2 Mouse mAb (2D10), liquid, clone 2D10, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

2D10, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... HMGA2(8091)

Analysis Note

Positive Control
HeLa cells

Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

Disclaimer

Toxicity: Regulatory Review (Z)

General description

Anti-HMGA2, mouse monoclonal, clone 2D10, recognizes the HMGA2 protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the HMGA2 protein.
Recognizes the HMGA2 protein in HeLa cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 1-93 human HMGA2, expressed as a GST fusion protein

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.9.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S Emmrich et al.
Leukemia, 28(5), 1022-1032 (2013-11-26)
MicroRNAs (miRNAs) play a pivotal role in the regulation of hematopoiesis and development of leukemia. Great interest emerged in modulating miRNA expression for therapeutic purposes. In order to identify miRNAs, which specifically suppress leukemic growth of acute myeloid leukemia (AML)

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