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Merck

FCMAB129CP

Milli-Mark Anti-EGFR-PerCP Antibody, clone LA22

clone LA22, Milli-Mark®, from mouse

Synonym(s):

Receptor tyrosine-protein kinase ErbB-1

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog

cell growth inhibiting protein 40

cell proliferation-inducing protein 61

epidermal growth factor receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

conjugate

Peridinin-Chlorophyll-Protein Complex

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LA22, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG2a

NCBI accession no.

NP_005219.2

UniProt accession no.

P00533

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

General description

180 kDa Observed
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen

A431 membrane proteins
Epitope: Unknown

Application

Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl
Milli-Mark Anti-EGFR-PerCP Antibody, clone LA22 detects level of EGFR & has been published & validated for use in FC.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).

Physical form

Protein G Purified
Purified mouse monoclonal IgG2a conjugated to PerCP in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8 oC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
A431 cells.
Evaluated by flow cytometry using A431 cells.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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