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Merck

FCMAB133F

Milli-Mark® Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

clone TRA-2-49/6E, Milli-Mark®, from mouse

Synonym(s):

Anti-TRA-2-49 FITC, Clone TRA-2-49 Antibody, TRA-2-49 Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Milli-Mark® Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate, clone TRA-2-49/6E, Milli-Mark®, from mouse

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TRA-2-49/6E, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Related Categories

Physical form

Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein A purified

Preparation Note

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Immunogen

2102Ep human Embryonal Carcinoma Cells
Epitope: Unknown

Analysis Note

Control
2102 Ep cells.
Evaluated by flow cytometry using 2102 Ep cells.

Application

Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

Antibody recognizes TRA-2-49.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

212/80/55 kDa Observed
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
FCMAB133F04053252509797

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