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Merck

FCMAB189F

Milli-Mark® Anti-CD25-FITC Antibody, clone ACT-1

clone ACT-1, Milli-Mark®, from mouse

Synonym(s):

Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, p55, TAC antigen

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ACT-1, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

NP_000408

UniProt accession no.

P01589

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IL2RA(3559)

General description

55 kDa (Uchiyama, 1981)
The interleukin-2 receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2 (Ishii, 1995). The 55 kDa α-chain is called CD25. CD25 is strongly expressed on the surface of activated T cells (Uchiyama, 1981), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide (Sugamura, 1997). In normal peripheral blood a variable expression on lymphoid cells (about 3-25%) is observed. The specificity of Anti-CD25, ACT-1, is identical to that of CD25-clustered antibodies (Schwarting, 1987; Aziz, 1996).

Immunogen

Phytohemagglutinin-stimulated blood lymphocytes

Application

This Milli-Mark Anti-CD25-FITC Antibody, clone ACT-1 is validated for use in FC for the detection of CD25.

Biochem/physiol Actions

Antibody recognizes Human CD25 antigen.

Analysis Note

Evaluated by flow cytometry using human lymphocytes

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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