FCMAB189F
Milli-Mark® Anti-CD25-FITC Antibody, clone ACT-1
clone ACT-1, Milli-Mark®, from mouse
Synonym(s):
Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, p55, TAC antigen
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
eCl@ss:
32160702
Conjugate:
FITC conjugate
Clone:
ACT-1, monoclonal
Application:
FACS
Citations:
-
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ACT-1, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IL2RA(3559)
General description
55 kDa (Uchiyama, 1981)
The interleukin-2 receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2 (Ishii, 1995). The 55 kDa α-chain is called CD25. CD25 is strongly expressed on the surface of activated T cells (Uchiyama, 1981), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide (Sugamura, 1997). In normal peripheral blood a variable expression on lymphoid cells (about 3-25%) is observed. The specificity of Anti-CD25, ACT-1, is identical to that of CD25-clustered antibodies (Schwarting, 1987; Aziz, 1996).
Immunogen
Phytohemagglutinin-stimulated blood lymphocytes
Application
This Milli-Mark Anti-CD25-FITC Antibody, clone ACT-1 is validated for use in FC for the detection of CD25.
Biochem/physiol Actions
Antibody recognizes Human CD25 antigen.
Analysis Note
Evaluated by flow cytometry using human lymphocytes
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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