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Merck

FCMAB294F

Milli-Mark® Anti-Ubiquitin Antibody, Lys48-Specific-FITC, clone Apu2

clone Apu2, Milli-Mark®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Milli-Mark® Anti-Ubiquitin Antibody, Lys48-Specific-FITC, clone Apu2, clone Apu2, Milli-Mark®, from rabbit

biological source

rabbit

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Apu2, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG

UniProt accession no.

P0CG47

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBB(7314)

Analysis Note

Control
HeLa cells
Evaluated by flow cytometry using HeLa cells

Application

Milli-Mark Anti-Ubiquitin Antibody, Lys48-Specific-FITC, clone Apu2 is an antibody against Ubiquitin for use in FC.
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Biochem/physiol Actions

Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Molecular weight varies
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Immunogen

Epitope: Lys48 ubiquitination site
Immunogen used was di-ubiquitin linked at Lys48

Physical form

Protein A purified
Purified recombinant rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Amelia B Karlsson et al.
Molecular biology of the cell, 25(8), 1355-1365 (2014-02-14)
Troyer syndrome is an autosomal recessive hereditary spastic paraplegia (HSP) caused by frameshift mutations in the SPG20 gene that results in a lack of expression of the truncated protein. Spartin is a multifunctional protein, yet only two conserved domains--a microtubule-interacting

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