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Merck

GF06

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3)

liquid, clone 134A-2B3, Calbiochem®

Synonym(s):

Anti-Transforming Growth Factor-α

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

134A-2B3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TGFA(7039)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa denatured and native forms of TGFα protein.
Recognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).

Immunogen

Human
recombinant, human TGF-α (~6 kDa mature form)

Application

Immunoblotting (1-5 µg/ml, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Preparation Note

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).

Analysis Note

Negative Control
NIH3T3 cells
Positive Control
A2058 or ST-FeSV FRE cells

Other Notes

Sorvillo, J.M., McCormack, E.S. 1990. Oncogene5, 377.
Coffey, R.J., et al. 1987. Nature328, 817.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
This antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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