Key Documents
GR18
Anti-Progesterone Receptor (Ab-1) Mouse mAb (PR4-12)
liquid, clone PR4-12, Calbiochem®
Synonym(s):
Anti-PR
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
PR4-12, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
feline, chicken, human, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody. Recognizes the ~94 kDa progesteron receptor A and the ~120 kDa progesterone receptor B.
Recognizes the ~94 kDa progesterone receptor A and the ~120 kDa progesterone receptor B in T47D cells and human breast carcinoma tissue.
This Anti-Progesterone Receptor (Ab-1) Mouse mAb (PR4-12) is validated for use in Immunoblotting, Paraffin Sections for the detection of Progesterone Receptor (Ab-1).
Immunogen
Human
recombinant, human progesterone receptor
Application
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.5-1 µg/ml, see comments)
Paraffin Sections (0.5-1 µg/ml, see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
HBL 100 cells
HBL 100 cells
Positive Control
T-47D cells or human breast carcinoma tissue
T-47D cells or human breast carcinoma tissue
Other Notes
Menendez-Botet, C.J. and Schwartz, M.K. J. 1992. Int. Fed. Clin. Chem.4, 94.
Clarke, R., et al. 1991. Nuclear Hormone Receptors,297, Academic Press, New York.
Green, S. and Chambon, P. 1991. Nuclear Hormone Receptors.,15, Academic Press, New York.
Wahli, W. and Martinez, E. 1991. FASEB J.5, 2243.
Beato, M. 1989. Cell56, 335.
Evans, R.M. 1988 Science240, 889.
Wittliff, J.L. 1988. in Cancer of the Breast. (Donegan, W. L. and Spratt, J.S. eds. et al.) p303. Saunders Philadelphia.
Clarke, R., et al. 1991. Nuclear Hormone Receptors,297, Academic Press, New York.
Green, S. and Chambon, P. 1991. Nuclear Hormone Receptors.,15, Academic Press, New York.
Wahli, W. and Martinez, E. 1991. FASEB J.5, 2243.
Beato, M. 1989. Cell56, 335.
Evans, R.M. 1988 Science240, 889.
Wittliff, J.L. 1988. in Cancer of the Breast. (Donegan, W. L. and Spratt, J.S. eds. et al.) p303. Saunders Philadelphia.
No pre-treatment required for staining human paraffin sections but using the pressure cooker method may enhance staining. Trypsin is required for rat or chicken paraffin sections. Trypsin/saponin is required for feline paraffin sections. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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