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Merck

IM29L

Anti-PAI-1 (Ab-1) Mouse mAb (2)

lyophilized, clone 2, Calbiochem®

Synonym(s):

Anti-Plasminogen Activator Inhibitor-1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Product Name

Anti-PAI-1 (Ab-1) Mouse mAb (2), lyophilized, clone 2, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

2, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Immunoblotting (1 µg/mL)
Immunoprecipitation
Immunocytochemistry (s
Neutralization Studies )

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SERPINE1(5054)

Analysis Note

Positive Control
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see application references)

Immunocytochemistry (see application references

Neutralization Studies (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.
Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.
This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).

Immunogen

Human
partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Legal Information

Sold under license of U.S. Patent 5,422,245.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Antibody should be titrated for optimal results in individual systems.
Janicke, F., et al. 1994. Cancer Research.54, 2527.
Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.
Nagayama, M., et al. 1994. Cancer73, 1398.
Petersen, H., et al. 1994. Cancer Res.54, 120.
Keijer, J., et al. 1991. Blood78, 401.
Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Preparation Note

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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