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MAB10750

Sigma-Aldrich

Anti-LIMK1 Antibody, clone 40/00-1B11-1-1

clone 40/00-1B11-1-1, from rat

Synonym(s):

LIM domain kinase 1, LIM motif-containing protein kinase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

40/00-1B11-1-1, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_010717.2

UniProt accession no.

P53668

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LIMK1(3984)

General description

LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities.
~ 73 kDa

Immunogen

Epitope: Unknown
GST-tagged recombinant protein corresponding to a region of mouse LIMK1.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-LIMK1 Antibody, clone 40/00-1B11-1-1 is validated for use in WB, IP, IC, IH for the detection of LIMK1.

Biochem/physiol Actions

The antibody recognizes LIMK1.

Physical form

Format: Purified
Protein G Purified
Purified in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PC3 cell lysate
Evaluated by Western Blot in PC3 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected LIMK1 on 10 µg of PC3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kazuhito Toyo-oka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(36), 12168-12181 (2014-09-05)
During brain development, neural progenitor cells proliferate and differentiate into neural precursors. These neural precursors migrate along the radial glial processes and localize at their final destination in the cortex. Numerous reports have revealed that 14-3-3 proteins are involved in

Global Trade Item Number

SKUGTIN
MAB1075004053252465277

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