Key Documents
MAB10764
Anti-SBP-tag Antibody, clone 20 | MAB10764
clone 20, 1.0 mg/mL, from mouse
Synonym(s):
streptavidin binding peptide
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
20, monoclonal
species reactivity
human
concentration
1.0 mg/mL
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
General description
Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.
The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa
Immunogen
Epitope: Unknown
Linear peptide corresponding to SBP tag.
Application
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Biochem/physiol Actions
Demonstrated to react with cells expressing the SBP tag.
This antibody recognizes SBP tag.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
pTrAP-GFP transfected Hek293 lysate
pTrAP-GFP transfected Hek293 lysate
Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.
Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.
Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB10764 | 04053252478697 |
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