MAB1096

Anti-Tumor Necrosis Factor-α Antibody, clone 195

Name: Anti-Tumor Necrosis Factor-α Antibody, clone 195
Brand: MM

clone 195, Chemicon^®, from mouse

Synonym(s):

TNF-alpha

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Tumor Necrosis Factor-α Antibody, clone 195, clone 195, Chemicon^®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

195, monoclonal

species reactivity

human

should not react with

pig, monkey, canine, mouse, rat, baboon

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon^®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

isotype

IgG3

NCBI accession no.

NM_000594.2

UniProt accession no.

P01375

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TNF(7124)

Related Categories

Small Pack Size Antibodies

Application

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.

Western Blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

The antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

SPECIES REACTIVITIES:

Not mouse, rat, canine, porcine, baboon or rhesus-monkey.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human, recombinant tumor necrosis factor-alpha produced in E. coli.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1114

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tumor necrosis factor-α and interferon-γ stimulate MUC16 (CA125) expression in breast, endometrial and ovarian cancers through NFκB.

Micaela Morgado et al.

Oncotarget, 7(12), 14871-14884 (2016-02-27)

Transmembrane mucins (TMs) are restricted to the apical surface of normal epithelia. In cancer, TMs not only are over-expressed, but also lose polarized distribution. MUC16/CA125 is a high molecular weight TM carrying the CA125 epitope, a well-known molecular marker for

TNF-α is a novel target of miR-19a.

Mei Liu et al.

International journal of oncology, 38(4), 1013-1022 (2011-01-29)

Many studies have demonstrated the overexpression and amplification of the miR-17-92 cluster in malignant human cancers, including B-cell lymphomas and lung cancers. The purpose of this study was to investigate for the first time, the expression of the miR-17-92 cluster

Prolactin and proinflammatory cytokine expression at the fetomaternal interface in first trimester miscarriage.

Emanuele Garzia et al.

Fertility and sterility, 100(1), 108-115 (2013-04-02)

To investigate the expression of prolactin (PRL), PRL-receptor (PRL-R), and the TH1 cytokines interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) at the maternofetal interface. Case-control study. University hospital unit of gynecology and obstetrics and research laboratories. Women undergoing

Global Trade Item Number

SKU	GTIN
MAB1096	04053252267505
MAB1096-25UG	04054839342646

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