MAB1285F

Anti-HLA-B27,Cln HLA-ABC-m3 FITC (mouse monoclonal)

Name: Anti-HLA-B27,Cln HLA-ABC-m3 FITC (mouse monoclonal)
Brand: MM

clone HLA-ABC-m3, Chemicon^®, from mouse

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

eCl@ss:

32160702

Product Name

Anti-HLA-B27,Cln HLA-ABC-m3 FITC (mouse monoclonal), clone HLA-ABC-m3, Chemicon^®, from mouse

biological source

mouse

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

HLA-ABC-m3, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon^®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG2a

NCBI accession no.

NM_005514.5

UniProt accession no.

P01889

shipped in

wet ice

target post-translational modification

unmodified

Application

Anti-HLA-B27 Antibody, clone HLA-ABC-m3, FITC conjugated detects level of HLA-B27 & has been published & validated for use in FC & IF.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

Flow cytometry and immunofluorescence 1:15 to 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

Biochem/physiol Actions

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BIOCHEMISTRY: pI = 8.0

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Immunogen

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 0.5ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

Preparation Note

Store at 2 to 8°C. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKU	GTIN
MAB1285F	04053252315794

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